BIOXAS-IMAGING

  Normal Operation

Designed for experiments that require greater/equal resolution to 10 μm. There are two stage-configuration available depending on the sample thickness. The 45 degrees stage-configuration with respect to the incident beam are generally set up for cross-sections varying from a few μms to a couple of hundreds of μms. For thicker samples reaching mm range, the 90 degrees stage-configuration is used.

Specifications

  • Beam focused by KB mirrors
  • Step-based or bi-directional fly imaging
  • Sample stage at either 90 or 45 degress to the incoming beam
  • Keyence camera with long working distance (85 mm), high zoom (variable) lens for sample visualization
  • Samples in ambient air or He gas box
  • Cryo Jet/LN2 Linkam for sample cooling (to be commissioned)

Beam size and flux

Beam spot sizes measured at 45 degrees (meaning ~1.41X increase in beam H size) and at 10 keV (5th harmonics):

  • 10 μm (H) x 5 μm (V) flux 3.2 x 10^11 ph/s/100 mA (full focused beam limited only by the acceptance of the KB mirrors)
  • 6 μm (H) x 5 μm (V) flux 1.5 x 10^11ph/s/100 mA (beam cut horizontally at the secondary source)

Setups

Stage configuration set at 90 degrees 

  • Micro-stage set at 90 degree orientation with and 3E Vortex detector at 45 degrees to the incident beam.

Micro90_a.png

Stage configuration set at 45 degrees

Micro45_a.png

Techniques

  • X-ray fluorescence imaging (XFI)
  • X-ray absorption spectroscopy (XAS)
  • X-ray absorption spectroscopy in situ (u-XAS)

Examples

  • Stage configuration at 45 degrees to the incident beam
  • The imaging data were collected at a resolution of 5 um at 100 ms dwell time

Oat.png

Distribution of Micronutrients in Arborg Oat (Avena sativa L.) Using Synchrotron X-ray Fluorescence Imaging (Deng et al., 2023).